Skip to main content
. Author manuscript; available in PMC: 2023 Sep 1.
Published in final edited form as: Adv Biol (Weinh). 2022 Apr 7;6(9):e2200019. doi: 10.1002/adbi.202200019

Figure 4. CGRP receptor expression in oral cancer cells.

Figure 4.

RT-qPCR was used to quantify relative RAMP1 and CALCR1 gene expression in A) human oral cancer cell lines (HSC3, SCC-4) and dysplastic (DOK) cells compared to non-tumorigenic skin keratinocytes (HaCaT) as well as (B) mouse oral cancer cell lines (MOC1, MOC2). Data is presented as the relative gene expression (2−ΔCT) normalized to ACTB and represents three different cell passage determinations of gene expression. Two-way ANOVA **p<0.01. C) Representative images of RAMP1-IR (green) in HaCaT, HSC3, and MOC1 cell lines. DAPI (blue) was used to label cell nuclei. D) Cell proliferation assays were used to determine change in cell reproduction after αCGRP stimulation. The DAPI-labeled cell counts of non-stimulated vehicle-treated cells were set as 1, and the percent change in cell number of treated cells was determined relative to vehicle-treated cells (0.0005% water). CGRP8–37 and AC187 blocker treatment indicates co-treatment with 0.3nM αCGRP and 1nM antagonist. Data represent the mean (±SEM) of three different cell passage determinations of cell proliferation. One-way ANOVA within cell line, p>0.05.