Figure 7. Genotype-dependent Oxtr mRNA and OXTR protein mismatch in substantia nigra pars reticulata.
OXTR protein (black; a-d) was labeled with autoradiography and, in adjacent sections, Oxtr mRNA (red; e-h) was labeled with in situ hybridization against a Hematoxylin counterstain (purple) in High-Oxtr voles (a,c,e,g) and Low-Oxtr voles (b,d,f,h). High-Oxtr and Low-Oxtr genotypes predicted protein binding in the nucleus accumbens (NAc) and substantia nigra pars reticulata (SNR), and mRNA expression in NAc. However, no Oxtr mRNA was detected in the SNR of either genotype. CPu, caudate putamen; ACC, anterior cingulate cortex; Tu, olfactory tubercle; IS, insular cortex. Scale bars for (a-d) =1mm shown in (d), for (e-h) =400μm shown in (h).