Fig. 2. Molecular signatures of the malignant cells and association with pathological characteristics in SBA.

a UMAP plot exhibiting the cell identification by CNV values in the STRT and 10× datasets. b, c Boxplots in the upper panel showing average normalized expression of the malignant cells and normal epithelial cells. Dot plots in the lower panel showing the log₂ fold change (FC) of malignant cell relative to normal epithelial cell of each patient or dataset. d Representative IHC staining of MUC1 in adjacent normal and primary tumor tissues (original magnification 100×). The rows of the paired normal and tumor samples are from the same patients, and three individual patients are listed. Scale bar, 100 μm. e Dot plot showing the log odd ratio values of tumor/normal of corresponding TFs, with P values calculated by χ² test. f Violin plot showing the expression scores of absorptive and transport gene sets in malignant and normal epithelial cells from different intestinal regions. g Heatmap showing the expression scores of metabolism pathways in malignant and normal epithelial cells from different intestinal regions.