Fig. 1. FBXW7 silencing induces senescence in cancer cells harboring wild-type p53 along with activation of both p53/p21 and p16INK4a/Rb pathways.
A549, HCT116, and MCF7 cells were infected with a lentivirus expressing shFBXW7 or shGFP, selected by puromycin for seven days, and subjected to senescence-associated β-galactosidase (SA-β-Gal) staining, followed by microscopy (A left) or immunoblotting (IB) with the indicated antibodies (B). The percentage of SA-β-Gal-positive cells in the total number of cells was determined (A right). Scale bars represent 50 μm. Data are presented as the mean ± standard deviation (SD). ***p < 0.001.