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. 2022 Sep 14;5:915. doi: 10.1038/s42003-022-03876-x

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Visceral insensitivity was maintained up to 6 months after surgery among LS lesioned mice, which showed a complete abolition of VMR to rectal and bladder electrical stimulation compared to sham mice. Tail and limb VMR responses showed no differences. P values refer to independent samples t tests (two-tailed). Statistically, there was no difference in VMR (P = 0.060) for rectal stimulation, while there was a significant (P = 0.029) for bladder stimulation. The lack of significance for rectal stimulation is likely attributable to the variability in sham EMG values, since VMRs were absent from rectum in DRG-removed mice. All error bars represent mean ± SD, individual markers represent individual animal averages.