Fig. 1. Atherogenic HFHCD induces a specific low-grade inflammation.

C57BL/6J mice were fed for 2 weeks with chow diet (CD), high fat high cholesterol diet (HFHCD) or obesogenic diet (60% HFD). a Plasmatic cholesterol measured by colorimetric assay. b Mouse weight variation (left) and spleen weight (right). c–e Flow cytometry analysis c in blood d, e in spleen. Splenocytes were incubated with Golgistop™1X/GolgiPlug ™ for 4 h in complete medium before flow analysis of % IFNγ, TNFα, IL-17 in CD4⁺ and CD8⁺ T cells. (gMFI: geo Mean fluorescence intensity). Data are expressed as mean ± s.e.m. n = 6 mice/group, one-way ANNOVA with Tukey’s multiple comparison test. Source data are provided as a Source Data file.