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. 2022 Sep 14;13:5399. doi: 10.1038/s41467-022-33034-0

Fig. 3. HFHCD favors migration of myeloid cells into the tumor.

Fig. 3

C57BL/6J mice were fed for 2 weeks with chow diet (CD) or high fat high cholesterol diet (HFHCD), then grafted with B16-F10 melanoma cells. a Cytokines and chemokines were measured by bead-based multiplex immunoassay (n = 4 mice/group) or Elisa (n = 5 in CD group and n = 4 in HFHCD group) in total B16-F10 tumor in CD or HFHCD mice at day 15 after tumor graft. b In vivo labeling of monocytes and neutrophils at day 8 after tumor graft: anti-Ly6G and anti-CD115 antibody were injected i.v. Six hours after injection, labeled cells were identified in blood and tumors by flow cytometry. Representive dot plots were showed. c, d Adoptive transfer of Ly6Chi monocytes from CD45.1 donor mice fed with CD or HFHCD into CD45.2 recipient mice bearing B16-F10 tumor fed with CD or HFHCD. c Experimental design. d Quantification of CD45.1 Ly6Chi monocytes in tumors and spleen 18 h after monocyte transplantation into CD45.2 recipient mice. Representative flow cytometry large dot plots illustrating gating of CD45.2 and CD45.1 among CD11b + Ly6Chi cells in tumors HFHCD. Data are expressed as mean±sem. Analysis of difference within groups were performed with two-sided Mann–Whitney t-test. Source data are provided as a Source Data file.