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. 2022 Sep 1;16:960149. doi: 10.3389/fnins.2022.960149

FIGURE 1.

FIGURE 1

Expression patterns of rnf2 in zebrafish embryos. (A) Whole mount in situ hybridization (WISH) of rnf2 at the indicated time points. (B) The expression of rnf2 at 72 hpf WT embryos. Black arrows showing the expression of rnf2 in the gut of zebrafish embryos. (C) Immunohistochemistry images of Rnf2 and HuC/D in zebrafish gut (40 × oil, embryos direction: anterior is to the left). The overlapping of Rnf2 and HuC/D expression is marked by the white box. (D) Quantitation of percentage of Rnf2/HuC double positive cells (n = 29). The experiments were repeated at least three times. Scale bar: 0.1 mm.