FIGURE 3.

Fibulin‐4 promotes invasion and migration but inhibits apoptosis in esophageal squamous cell carcinoma (ESCC) cells. (a) Eca109 or KYSE30 cells that migrated or invaded across the membrane were fixed and stained. (b) Cells that passed through the membrane were counted in three random fields. (c) Eca109 and KYSE30 cells treated or left untreated were analyzed by flow cytometry. The percentage of PE‐ and/or 7‐AAD‐positive cells is included in the panels. (d) The results are expressed as the percentages (mean standard deviation [SD] of three repeated experiments) of apoptotic/necrotic cells. The error bars indicate the means ± SD averaged from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. NC: Negative control of silencing lentiviral vector; vector: Negative control of overexpression lentiviral vector.