Phenotypes and genetic complementation of a
tadC mutant. Top panels, mutant strain Aa1359
(tadC::IS903φkan). Middle panels,
strain Aa1359 containing the pJAK16 vector plasmid. Bottom panels,
Aa1359 containing pJAK16 with the cloned tadC ORF from
CU1000N. The methods used are identical to those described for Fig. 1.
Results for complementation of the other mutants were essentially the
same (Table 1). Bars, 1.0 mm (a, d, and g), 0.2 μm (b and e), and 0.5
μm (h).