Fig. 8.

Silencing GOLM1 expression in Huh-7 cells modulates cell proliferation, apoptosis, and cell cycle. A: CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTT) to measure cell proliferation in GOLM1-silenced Huh-7 cells at 24, 48, and 72 h. B: [³H] thymidine incorporation assay to measure proliferation. C: DAPI nuclear staining of GOLM1-depleted and control Huh-7 cells grown on coverslips. Coverslip slides were scanned using 3D HISTECH Panoramic 250 Flash III, and snapshots were taken at 0.8× magnification using CaseViewer, version 2.2. The scale bar represents 2,000 μm. D: RealTime-GloTM Annexin V Apoptosis Assay to measure apoptosis from 24 to 88 h in GOLM1-silenced cells. E: Cell cycle analysis of GOLM1 knockdown and control cells. Data are represented as mean ± SD, all experiments were repeated at least three times with multiple replicates in each set. ∗∗∗P < 0.001 and ∗∗P < 0.01. DAPI, 4′,6-diamidino-2-phenylindole.