Fig. 9.

GOLM1 depletion alters the expression of genes involved in ceramide synthesis or encoding Golgi proteins. A: mRNA expression of genes involved in sphingolipid metabolism. B: mRNA expression of sphingolipid biosynthesis regulators. C: Western blot of ORMDL3 in NT siRNA and siGOLM1-transfected Huh-7 cells (dashed [---] line represents NT siRNA). Data are represented as mean ± SD, all experiments were repeated at least three times with multiple replicates in each set. ∗∗∗P < 0.001, ∗∗P < 0.01, and ∗P < 0.05. A representative full Western blot is available in supplemental data section.