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. 2022 Sep 7;24(5):662. doi: 10.3892/etm.2022.11598

Figure 3.

Figure 3

SIRT3 in DACs contributes to neuroprotection via mitochondrial apoptotic pathway. (A) Protein levels of Bax, Bcl-2, cleaved caspase-3, Mito-Cyt C and Cyto-Cyt C in DACs from the SIRT3 group, Vector group, and Control group were measured using western blotting. (B) Protein levels of Bax, Bcl-2, cleaved caspase-3, Mito-Cyt C and Cyto-Cyt C in DACs from the siSIRT3 group, Scrambled group, and Control group were determined using western blotting. Representative western blot (left panel) and quantification (right panel) of Bax, Bcl-2, Cyt C and cleaved caspase-3 protein expression in DACs transfected with scrambled siRNA or siSIRT3 for 48 h. The spectrophotometric method was used to detect caspase-3 and caspase-9 enzyme activities in DACs transfected with SIRT3 plasmid or empty vector (C), and scrambled siRNA or SIRT3 siRNA (D) for 48 h. Results are normalized to those of the Control group. Data are presented as mean ± S.D. n=4. *P<0.05 vs. Control; #P<0.05 vs. Vector or Scrambled. SIRT3, sirtuin 3; DAC, dopaminergic neuronal cell; si/siRNA, small interfering RNA; Mito-Cyt C, mitochondrial cytochrome c; Cyto-Cyt C, cytoplasmic cytochrome c.