TABLE 1.
PCR amplification of the 4,451-bp Rg-1 deletion marker and the rtfA gene from Rg variants of M. avium strain 2151a
| Rough variants | 4,451-bp PCR product | rtfA gene |
|---|---|---|
| Rg-1b | + | − |
| Rg-4b | − | + |
| Rg-O1c | + | − |
| Rg-O2c | + | − |
| Rg-O3c | + | − |
| Rg-O4c | + | − |
| Rg-O5c | + | − |
| Rg-O6c | + | − |
| Rg-O7c | + | − |
| Rg-O8c | − | − |
| Rg-O9c | + | − |
| Rg-O10c | + | − |
| Rg-T1d | − | + |
| Rg-T2d | − | + |
| Rg-T3d | − | + |
| Rg-T4d | − | + |
| Rg-T5d | + | − |
a
The 4,451-bp PCR product was amplified using primers PL1 and PR1. rtfA was amplified using protocols described by Eckstein et al. (12).
b
Rg variants originally described by Belisle et al. (4).
c
Rg variants derived in this study from the SmO morphotype.
d
Rg variants derived in this study from the SmT morphotype.