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. 2022 Aug 29;12(14):6422–6436. doi: 10.7150/thno.77350

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Protein expression initiated by circRNA and linear mRNA. (A) Upper panel, a scheme of the elements needed for circRNA^D2GFP production via the PIE system. Lower panel, sanger sequencing chromatograph for the junction site of the reverse-transcribed circRNA^D2GFP sample. Protein expression level of circRNA^D2GFP and the D2GFP-coding linear mRNA (mRNA^D2GFP) in (B) HEK293T cells and (C) NIH3T3 cells. Statistical analysis of the percentage of GFP positive (D) HEK293T cells and (E) NIH3T3 cells at different time points. *p < 0.05.