Figure 3.

Mitochondria-targeted hybrid nanozymes as superoxide scavengers protect heart function during IR injury. (A) Mito-Fenozyme were fabricated by in situ synthesis of MnO₂ into FTn core via Mn²⁺ oxdation in the presence of H₂O₂, followed by TPP-NHS ester conjugation with free -NH₂ of protein. (B) TEM images of FTn protein shell (negative staining with 1% uranyl acetate), MnO₂ and Mito-Fenozyme. (C) Schematic of intracellular conversion of free radicals to noncytotoxic molecules under Mito-Fenozyme. (D) Confocal images (upper) and quantification analysis (bottom) of the effect of MnO₂ Fenozyme and Mito-Fenozyme on mitochondrial oxidative injury by mitoSOX as O^2•- indicator. Scale bar = 10 µm. (E) Representative images of midpapillary regions of the hearts, 14 d after IR using Masson's trichrome staining. Blue, collagen-rich scar tissue; red, viable myocardium. The scar tissue was indicated with dashed box. Adapted with permission from ⁹⁷, copyright 2021, John Wiley & Sons, Inc.