Figure 4.
Cell-like delivery system (ASSP-MP) for improved vascular regeneration in MI model. (A) ASSP-MPs were fabricated by loading proangiogenic bioactive factors collectively known as“cocktail” factors (CFs) secreted from SSPs into PLGA-MPs, and then PLGA-MPs were coated with cell membrane of SSPs to form ASSP-MPs. (B) Heatmap of proteins differentially expressed in 2D-CF and 3D-CF, based on LC-MS proteomic analysis. (C) Confocal imaging and (D) image-based quantification analysis of blood vessels in ischemic muscles by immunostaining with anti-CD31 antibody at 21 days after treatment. Scale bar = 50 µm. Adapted with permission from 27, copyright 2020, American Association for the Advancement of Science.