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. 2022 Jun 14;41(18):e109990. doi: 10.15252/embj.2021109990

Figure EV1. Purification and phylogenetic analyses of AI‐2 exporters from Escherichia coli .

Figure EV1

  • A–D
    Size exclusion chromatography (SEC) and BN–PAGE profiles for the four AI‐2 exporters from E. coli, TqsA (A), YdiK (B), YhhT (C), and PerM (D). The retention volumes for each of the proteins are included. Superose 6 increase 10/300 GE column was used for the final size exclusion step.
  • E
    Phylogenetic analysis of the four AI‐2 exporters and Upf0118 family using the maximum‐likelihood method. MEGAX was utilized for the analysis and the branch distances are mentioned on the respective branches of the tree.