Table 1.
Cryo‐EM data collection, refinement, and validation statistics.
| Specifications | TqsA (EMDB: 12256) (PDB:7NB6) | YdiK (EMDB: 13057) (PDB:7OT9) |
|---|---|---|
| Data collection and processing | ||
| Magnification | 105,000 | 105,000 |
| Voltage (kV) | 300 | 300 |
| Electron exposure (e−/Å2) | 80 | 80 |
| Defocus range (μm) | −1.1 to −2.1 | −1.1 to −2.1 |
| Pixel size (Å) | 0.837 | 0.831 |
| Symmetry imposed | C5 | C5 |
| Initial particle images (no.) | 1,218,552 | 1,222,739 |
| Final particle images (no.) | 134,300 | 619,311 |
| Map resolution (Å) | 3.35 | 2.80 |
| FSC threshold | 0.143 | 0.143 |
| Map resolution range (Å) | 3.0–7.2 | 2.6–6.5 |
| Refinement | ||
| Initial model used | – | – |
| Model resolution (Å) | 2.9 | 2.5 |
| FSC threshold | 0.143 | 0.143 |
| Map sharpening B factor (Å2) | −30 | −10 |
| Model composition | ||
| Non‐hydrogen atoms | 13,020 | 8,410 |
| Protein/nucleotide residues | 1,700 | 1,105 |
| Ligands | – | – |
| B factors (Å2) (min/max/mean) | ||
| Protein | 18.22/197.18/109.91 | 71.51/175.24/100.68 |
| Ligands | – | – |
| R.m.s deviations | ||
| Bond lengths (Å) | 0.010 | 0.006 |
| Bond angles (°) | 1.282 | 1.002 |
| Validation | ||
| MolProbity score | 2.13 | 1.90 |
| Clashscore | 13.42 | 9.94 |
| Poor rotamers (%) | 0.49 | 0 |
| CaBLAM outliers (%) | 2.38 | 2.39 |
| Ramachandran Plot | ||
| Favored (%) | 91.72 | 94.42 |
| Allowed (%) | 8.28 | 5.58 |
| Disallowed (%) | 0 | 0 |