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. 2022 Aug 10;41(18):e110488. doi: 10.15252/embj.2021110488

Figure 1. Basal layer cells express different LRC/non‐LRC preferred‐genes in scales vs. interscales of mouse tail skin.

Figure 1

  • A
    Model of tail skin organization in IFE domains.
  • B, C
    (B) Whole mount low magnification images show marking in interscale (K10+) vs. scale regions, quantified in (C). Error bars are SDs. The data shown are average of four different time points (2 week, 3 month, 6 month and 1 year) each consisting of two mice and five images per mice. P‐value is calculated for difference between scale vs. interscale using Student's t‐test (mixed Anova).
  • D
    Tail skin sections immunofluorescence stained for interscale (K10) or scale (K31) supra‐basal markers, and basal markers. Scale bar = 20 μm or 50 μm as indicated on the panels. Asterisks indicate that photoshop was used to erase the autoflourescent cornified layer. Dashed lines indicate ROI in basement membrane.
  • E
    Quantification of images like those in (D) with n = 3–4 mice and 6–7 images per mouse showing preferred‐gene enrichment. Error bars are SDs. P‐values calculated by Student's t‐test (mixed effect model).
  • F
    Relative gene expression/area levels from (D) and (E), normalized to sum to one, and displayed in a barycentric plot obtained from a total of 3–4 biological replicates and 6–7 images per replicate. Error bars are SEMs. The proportions in Fig 1F were computed using log ratios (Aitchison, 1982).
  • G
    Cartoon summarizing spatial distribution of markers in the tail IFE domains.