Figure 3. Single‐cell RNA‐seq of basal IFE LRCs/non‐LRCs sorted from mouse back and tail skin.

• A
  FACS sorting of basal LRCs, mid‐LRCs and non‐LRCs.
• B
  scRNA‐seq data of sorted cells from (A) analyzed for LRC/non‐LRC gene scores computed from microarray data (Sada et al, 2016).
• C, D
  UMAP cell clusters identified based on published markers and cell cycle regression analysis (see 3E). Marker definition analysis is described in Fig EV5I–M (back) and Appendix Fig S1C–E (tail).
• E
  Basal cluster definition markers.
• F
  Model of basal IFE domain cell states.
• G
  LRCs and non‐LRCs computed gene scores are differentially enriched in tail SC clusters. P‐values from pairwise Wilcoxon rank‐sum tests with Benjamini‐Hochberg correction.
• H, I
  Violin and feature plots of markers in tail skin without proliferative (Proli) clusters. Note LRC/interscale markers Sox6&Igfbp3 enriched in SC‐2&3 and non‐LRC/scale markers K15/Sostdc1 enriched in SC‐1. Slc1a3 is in SC‐1 (scale); SC‐2 expression is likely due to Slc1a3 expression in interscale line.
• J
  Model of LRC/non‐LRC gene scores and marker expression in scRNA‐seq clusters found in tail vs. back skin.