TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype and/or relevant characteristics | Reference or source |
|---|---|---|
| E. coli | ||
| S17.1 | thi pro hsdR RP4-2-tet::Mu-Kan::Tn7 Tra+ Trir Strr | 42 |
| S17.1 λpir | S17.1-derived recA λpir phage lysogen | 27 |
| E. carotovora subsp. carotovora | Prototroph | L. Corazza (MAF Institute of Phytopathology, Rome, Italy) |
| P. aeruginosa | ||
| PAO1 (ATCC15692) | Prototroph | American Type Culture Collection |
| PALS124 | pvdA | 44 |
| PAAC1 | pvdA::tet site-specific mutant | 42 |
| Pseudomonas strain B10 | Prototroph | 25 |
| B10CA1 | psbA::cat site-specific mutant | This study |
| Plasmids | ||
| pLAFR1 | Broad-host-range cosmid vector derived from IncP1 plasmid pRK290; RK2 replicon λcos+ rlx lacZα Mob+ Tra− Tcr | 25 |
| pJLM1 | 23.2-kb EcoRI genomic fragment of Pseudomonas strain B10 ligated to pLAFR1 | 25 |
| pUCP18 | E. coli-Pseudomonas shuttle vector derived from pUC18; pMB1 and pRO1600 replicon lacZα bla Apr Cbr | 42 |
| pACYC184 | p15A replicon; Cmr Tcr | 7 |
| pJP5603 | R6K-based suicide vector | 27 |
| pJP1 | 4.1-kb psbA::cat fragment ligated to the PstI-HindIII sites of pJP5603 | This study |
| pCAΔSh | 2.7-kb PstI-SphI fragment of pJLM1 ligated to pUCP18 | This study |