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. 2022 Sep 14;10(9):e004219. doi: 10.1136/jitc-2021-004219

Figure 2.

Figure 2

CD155 level in macrophages determined phenotypic transition and function. Expression pattern of CD155 in THP-1 cells, NC-CD155 transfected THP-1 (hCD155+), and sh-CD155 transfected THP-1 (hCD155–) cells analyzed by FACS (A), RT-qPCR (B), and Western blot analysis (C and D). (E–G) CD86 expression patterns in LPS+IFN-γ-treated hCD155+ and hCD155– macrophages. (H–J) CD206 expression patterns in IL-4+IL-13-treated hCD155+ and hCD155– macrophages (gated on CD68+ cells). (K) IL-12 and TNF-α protein expression patterns in LPS/IFN-γ-treated hCD155+ and hCD155– macrophages. (L) IL-10 and TGF-β protein expression patterns in IL-4/IL-13-treated hCD155+ and hCD155– macrophages. Data were presented as mean±SD, n=3. A significant difference between the groups, *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001. FACS, fluorescence activated cell sorter; IL, interleukin; IFN, interferon; LPS, lipopolysaccharide; mRNA, messenger RNA; ns, no significant difference; RT-qPCR, real time quantitative PCR; TGF, transforming growth factor; TNF, tumor necrosis factor.