FIGURE 3.

Individual genes that are differentially expressed by env-specific CD4 T cells expressing RV144 polyfunctional correlate profile compared with non-correlate env-specific CD4 T cells. (A) Violin plots of log counts of the scRNA sequences for the 14 significantly differentially expressed genes between the env-specific CD4⁺ T cells grouped by the cytokine protein expression profiles that correlated with reduced risk of HIV infection in RV144, labeled here as correlate (IL-4⁺, IL-2⁺, CD154⁺, and IFN-γ⁺ and IL-4⁺, IL-2⁺, and CD154⁺) or non-correlate. (B) Heatmap of Z scores of the differentially expressed genes clustered by T cells with correlate or non-correlate cytokine expression profile. Genes with FDR <0.05 and fold change of >1.25 or less than −1.25 were considered significantly differentially expressed between groups.