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. 2022 Sep 9;36(10):e22540. doi: 10.1096/fj.202200684RR

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© 2022 The Authors. The FASEB Journal published by Wiley Periodicals LLC on behalf of Federation of American Societies for Experimental Biology.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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Differentiation of primary human bone marrow mesenchymal stem cells (MSCs) in Stacks. (A) MSCs from two separate donors were seeded into Stacks and cultured for 14 days under control or adipogenic conditions. (B) Adipocytic differentiation was shown by the development of lipid droplets, identified with LipidSpot™ staining (green) and Hoechst nuclear staining (blue) by confocal microscopy. (C) LipidSpot™ ‐positive cells and total nucleated cells were quantified using automated image analysis and expressed as a percentage of LipidSpot™‐positive cells. Each data point represents percentage of LipidSpot‐positive cells in one microwell; *p < .05.