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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: Curr Protoc. 2022 Aug;2(8):e514. doi: 10.1002/cpz1.514

Table 1.

Plasmid amounts for transfection into one 15 cm plate to produce recombinant lentivirus

Plasmid: μg to be added Percentage
pCMV-VSV-G 3.38 15
psPax2 8.44 37.5
Transgene cassette (e.g. pLKO-cell-mEm) 10.7 47.5
Total: 22.5