FIG. 2.

(A) Two-dimensional Southern blotting of B31-RML plasmids hybridized with an eppA2-derived probe. Three forms of cp9-2 are evident and marked as CCC (covalently closed circular), OC (open circular), and Linear. (B) Diagram of cp9-2 indicating the locations of sequences complementary to oligonucleotides EA-G and EA-H, with 5′-to-3′ directions indicated by arrows. (C) Results of long-range PCR of B31-RML and B31-4a. The approximately 9-kb PCR product of cp9-2 is indicated by an arrow. A smaller PCR product was obtained from both cultures and is marked with an asterisk. Positions of DNA size standards (in kilobases) are indicated to the left of panels A and C.