Table 2.
Monitor ion pairs, CE, segment, repeatability and stability of multiple compounds in G. elegans samples.
| No. | Analytes | t/R (min) | Ion pair | CE/eV | Seg | Repeatability (mg/g ± RSD%) | Stability/RSD(%) |
|---|---|---|---|---|---|---|---|
| 1 | 7-Deoxygelsemide or 9-deoxygelsemide | 6.2 | 197.1 → 105.1 | 30 | 2 | 0.001 ± 3.31 | 3.02 |
| 2 | 11,14-Dihydroxygelsenicine | 11.4 | 359.2 → 108.1 | 30 | 3 | 0.086 ± 2.88 | 1.63 |
| 3 | 14,15-Dihydroxygelsenicine | 11.5 | 359.2 → 328.1 | 30 | 3 | 0.066 ± 2.96 | 2.56 |
| 4 | Unknown (375) | 15.6 | 375.2 → 311.1 | 30 | 4 | 0.029 ± 1.42 | 1.98 |
| 5 | Gelsemine | 16.5 | 323.2 → 70.1 | 33 | 4 | 3.787 ± 0.94 | 1.58 |
| 6 | 11-Hydroxygelsenicine | 16.5 | 343.2 → 281.1 | 30 | 4 | 0.017 ± 1.27 | 3.42 |
| 7 | Gelsemicine | 17.1 | 359.2 → 108.1 | 30 | 4 | 0.002 ± 2.79 | 2.12 |
| 8 | GSIR-1 | 17.4 | 183.1 → 91.1 | 30 | 4 | 0.001 ± 2.76 | 4.68 |
| 9 | 14-Hydroxygelsemicine or other hydroxylation of gelsemicine | 17.5 | 375.2 → 313.2 | 35 | 4 | 0.237 ± 2.36 | 1.56 |
| 10 | 14-Hydroxygelsedine | 17.8 | 345.2 → 285.1 | 30 | 4 | 0.034 ± 2.13 | 2.46 |
| 11 | 14-Hydroxygelsenicine | 18.0 | 343.2 → 108.1 | 30 | 5 | 0.730 ± 3.92 | 1.63 |
| 12 | Koumine | 19.1 | 307.2 → 180.0 | 53 | 5 | 0.830 ± 1.83 | 2.07 |
| 13 | 14-Dehydroxygelsefuranidine or other dehydroxylgelsefuranidine (2) | 19.4 | 405.2 → 343.1 | 30 | 5 | 0.071 ± 2.70 | 1.86 |
| 14 | 11-methoxy-14-hydroxygelsenicine | 19.5 | 373.2 → 108.1 | 30 | 5 | 0.142 ± 2.16 | 2.69 |
| 15 | Unknown (295) | 19.8 | 295.2 → 138.1 | 30 | 5 | 0.058 ± 1.78 | 3.03 |
| 16 | Hydroxyl of gelsedine | 19.9 | 345.2 → 285.1 | 30 | 5 | 0.001 ± 4.46 | 4.20 |
| 17 | Gelsemoxonine | 20.1 | 359.2 → 311.1 | 30 | 6 | 0.942 ± 3.38 | 2.10 |
| 18 | One of other 5 hit compounds | 21.1 | 371.2 → 323.1 | 30 | 6 | 0.067 ± 2.84 | 1.64 |
| 19 | 14-Dehydroxygelsefuranidine or other dehydroxylgelsefuranidine (1) | 21.1 | 405.2 → 374.2 | 30 | 6 | 0.049 ± 2.92 | 4.24 |
| 20 | Koumidine | 21.2 | 295.2 → 144.1 | 30 | 6 | 0.042 ± 2.01 | 2.06 |
| 21 | 14-Acetoxygelsedilam or other acetoxyl of gelsedilam (1) | 21.4 | 373.2 → 342.2 | 30 | 6 | 0.005 ± 4.13 | 2.06 |
| 22 | 11-Hydroxyhumantenine | 21.5 | 371.2 → 325.2 | 30 | 6 | 0.312 ± 1.77 | 2.26 |
| 23 | Gelsevirine | 22.0 | 353.2 → 291.2 | 30 | 7 | 0.191 ± 2.30 | 1.39 |
| 24 | Gelsenicine | 22.2 | 327.2 → 265.1 | 30 | 7 | 0.958 ± 1.75 | 1.41 |
| 25 | 12β-hydroxy-pregn-4,16-diene-3,20-dione | 22.2 | 329.2 → 97.1 | 30 | 7 | 0.002 ± 4.96 | 3.00 |
| 26 | Koumicine | 23.8 | 353.2 → 166.1 | 30 | 7 | 0.342 ± 3.81 | 1.70 |
| 27 | Gelsedine or Nb-Methylgelsedilam | 23.4 | 329.2 → 298.2 | 30 | 7 | 0.004 ± 1.54 | 2.01 |
| 28 | Gelseoxazolidinine | 23.9 | 429.2 → 339.2 | 30 | 7 | 0.003 ± 2.53 | 2.99 |
| 29 | 16-epi-voacarpine or gelsevirine N-oxide | 24.0 | 369.2 → 166.1 | 30 | 7 | 0.823 ± 1.20 | 1.84 |
| 30 | Humantenoxenine | 24.3 | 369.2 → 108.1 | 30 | 7 | 0.009 ± 3.48 | 3.31 |
| 31 | Iso-Gelsedine or Nb-Methylgelsedilam | 24.7 | 329.2 → 269.3 | 30 | 7 | 0.014 ± 4.55 | 2.48 |
| 32 | 1-O-Caffeoylquinic acid or 4-O-Caffeoylquinic acid | 25.0 | 355.1 → 135.0 | 30 | 8 | 0.117 ± 1.24 | 1.39 |
| 33 | Gelseziridine | 25.7 | 343.2 → 108.1 | 30 | 8 | 0.001 ± 2.57 | 2.27 |
| 34 | Na-Desmethoxyhumantenine | 25.8 | 325.2 → 136.1 | 35 | 8 | 0.020 ± 2.63 | 1.55 |
| 35 | 14-Acetoxygelsedilam or other acetoxyl of gelsedilam (2) | 26.6 | 373.2 → 342.2 | 30 | 8 | 0.033 ± 2.14 | 1.95 |
| 36 | 14-Acetoxygelsedilam or other acetoxyl of gelsedilam (3) | 27.3 | 373.2 → 342.2 | 30 | 8 | 0.033 ± 2.48 | 2.57 |
| 37 | 19R-Hydroxydihydrogelsevirine or 19S-Hydroxydihydrogelsevirine | 26.5 | 371.2 → 164.1 | 30 | 8 | 4.526 ± 4.24 | 4.18 |
| 38 | 11-Methoxyhumantenine | 26.8 | 385.2 → 339.2 | 30 | 8 | 0.061 ± 4.33 | 4.53 |
| 39 | iso-12β-Hydroxy-5α-pregn-16-ene-3,20-dione | 31.2 | 331.2 → 109.1 | 30 | 11 | 0.003 ± 4.42 | 4.02 |
| 40 | Gelse-norursane E | 31.7 | 471.2 → 217.1 | 30 | 10 | 0.008 ± 4.28 | 3.99 |
| 41 | 12β-Hydroxy-5α-pregn-16-ene-3,20-dione | 31.8 | 331.2 → 97.1 | 30 | 11 | 0.038 ± 4.24 | 4.18 |