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Effects of PRF on NAMPT and TLR4/NF-κB pathway in RAW 264.7 cells in vitro. A, Effects of PRF on expression of proteins TLR4, HMGB1, and p-p65; B, Effects of NAC/NMN cotreatments on PRF-induced inhibition on NAMPT and TLR4/NF-κB; C, Quantified results of experiment B. Data represent as mean ± SD, n = 6. Statistical significance: *P < 0.05 and **P < 0.01 compared with LPS-treated cells, #P < 0.05 and ##P < 0.01 compared with LPS + PRF treated cells.
