Fig. 2.
Effects of Chry on mitochondria function and cell viability toward HepG2 cells. (A) Determination of ΔΨm by TMRE probe. (B) Determination of ATP by ATP Assay Kit. (C) Determination of O2–·production by MitoSOX probe. (D) Determination of GSH/GSSG by GSH/GSSG Assay Kit. The concentrations and the action time were indicated on the figures. (E) Cell viability for 48 h was determined by MTT assay. (F) Analysis of cell death by nuclear condensation using Hoechst/PI staining. Scale bars of fluorescence images: 20 μmol/L. The values are expressed as the means ± SD; n = 3, *P ＜ 0.05 vs control.