Table 1.
List of primary and secondary antibodies used in immunofluorescent staining
|
Antibody name
|
Species
|
Dilution
|
Catalog number
|
Company
|
| Primary antibodies | ||||
| CK8 | Mouse | 1:200 | 904801 | Biolegend |
| CK19 | Rabbit | 1:200 | ab15463 | Abcam |
| CD44 | Mouse | 1:100 | sc-7297 | Santa Cruz Biotechnology |
| Ki67 | Mouse | 1:50 | sc-23900 | Santa Cruz Biotechnology |
| p- HistoneH2AX | Rabbit | 1:200 | #9718S | Cell Signaling |
| Secondary antibodies | ||||
| Alexa fluoro 488 | Goat anti-mouse | 1:200 | A-28175 | Invitrogen |
| Alexa fluoro 568 | Goat anti-rabbit | 1:200 | A-11011 | Invitrogen |
| Phalloidin | 1:200 | R415 | Invitrogen | |