TABLE 1.

Distribution of the inserted sequence among clinical E. coli strains

Strain or isolate(s)	Origin	PCR test 1a		PCR test 2b		Source or reference
		putP (202 bp)	Internal fragment (301 bp)	rpoS border (579 bp)	o454 border (483 bp)
ATCC25992	Clinical	+	+	+	+	G. Reid
BB593:2b	Fecal (child)	+	+	+	+	G. Reid
Co1	Fecal (non-UTI)	+	−	−	−	G. Reid (35)
SM47c	Neonatal meningitis	+	−	−	−	G. Reid
2 Isolatesd	Hemorrhagic colitis	+	−	−	−	C. L. Gyles
3 Isolates	Hemorrhagic colitis	+	−	NT	NT	C. L. Gyles
3 Isolatesd	Infantile diarrhea	+	−	−	−	C. L. Gyles
16 Isolates	Infantile diarrhea	+	−	NT	NT	C. L. Gyles
2 Isolates	Infantile diarrhea	+	+	+	+	C. L. Gyles
1 Isolatec	Catheter	+	−	−	−	G. Reid
3 Isolatesd	Catheter	+	−	−	−	G. Reid
3 Isolates	Catheter	+	+	+	+	G. Reid
431	Bacteriuria	+	+	+	+	G. Reid (35)
950	UTI (bacteremia)	+	+	+	+	G. Reid (38)
2239	UTI	+	+	+	+	G. Reid
C1212	UTI	+	+	+	+	G. Reid (31)
C1214	UTI	+	+	+	+	G. Reid (31)
2 Isolates	Cystitis	+	−	−	−	G. Reid
2 Isolatesd	Cystitis	+	−	−	−	G. Reid (33, 34)
8 Isolates	Cystitis	+	+	+	+	G. Reid (33, 34)
CFT073	Acute pyelonephritis	+	+	+	+	H. Mobley (27)
HU734	Acute pyelonephritis	+	+	+	+	G. Reid (16, 17)
5 Isolates	Pyelonephritis	+	+	+	+	G. Reid

^aMultiplex PCR test 1 was performed with putP primers plus primers C and D (Fig. 2). The putP primers were putP1, 5′-GGTTGCGTGTGCATACCGA-3′ (bp 287 to 305 of putP), and putP2, 5′-GCCGTTTCGTAGCTCATGC-3′ (bp 469 to 488 of putP). +, DNA fragments of the indicated sizes were obtained; −, PCR yielded no DNA fragment. 

^bMultiplex PCR test 2 was performed using primers A, B, E, and F (Fig. 2). +, DNA fragments of the indicated sizes were obtained; −, PCR yielded no DNA fragment; NT, PCR test 2 was not performed. 

^cMultiplex PCR test 2 yielded a single 526-bp DNA fragment (characteristic of E. coli K-12). 

^dMultiplex PCR test 2 yielded a DNA fragment pattern unlike that of E. coli CFT073 or E. coli MC4100.