Figure 1.

Schematic illustration of the locations of short (UL) and long (F) amplicon target regions along the HCMV genome of strain Merlin (NCBI: AY446894). The first track shows the overall HCMV genome structure that consists of a unique long (UL) and a unique short (US) region. Above, forward and reverse primers shown as triangles, and the size of the long amplicons (F5, F1, F3, and F4) are depicted. Next, a zoom-in into these four long amplicons (green horizontal lines with amplicon sizes) is shown separately. The mean pairwise nucleotide identity for over 200 published sequences for these regions is shown in three colours reflecting the height of the bars: green (100 per cent), brown (≥30 per cent to <100 per cent), and red (≤30 per cent). All annotated CDSs are shown as filled arrows, and those used for short- and long-read genotyping are in orange and green, respectively. Lastly, blue lines below each long amplicon indicate short amplicon regions (UL6, UL73, UL74, UL146, and UL139) and the respective amplicon sizes. Graphs were generated with Geneious Prime 2019.0.3.