FIGURE 3.

(A) Total label‐free SP‐IRIS particle analysis of 50–200 nm EVs captured on spots with antibodies specific for β1, CD47, α4, β3, CD63, CD81 and CD9 using Jurkat and JinB8 supernatants. (B) Fluorescent imaging of CD63⁺ EVs using CF647‐anti‐CD63, (C) CF555‐anti‐CD81 for CD81⁺ EVs and, (D) CF488‐anti‐CD9 for CD9⁺ EVs captured on β1, CD47, α4, β3, CD63, CD81 and CD9 antibody spots as shown in Panel A. Significant values were calculated using a two‐sample t‐test assuming equal variances for each capture spots (n = 3), and Asterisks value indicate p‐values: * = P > 0.05, ** = P > 0.0002, and *** = P > 0.00003