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. Author manuscript; available in PMC: 2022 Sep 15.
Published in final edited form as: Cancer Cell. 2020 Dec 17;39(1):96–108.e6. doi: 10.1016/j.ccell.2020.11.006

Figure 2. Type I IFN signal pathway is activated in dMLH1 tumor cells.

Figure 2.

(A) ISGs expression at the mRNA level in cultured cells (n=3) was determined by qPCR. Relative expression fold change of representative ISG (Isg15) was shown. (also see Figure S2A).

(B) Phosphorylation of STAT1 at Y701 was shown by WB.

(C) IFN-β was quantified by ELISA in the supernatant of indicated cell lines (n=3).

(D and E) Isg15 and IFN-β were quantified by qPCR and ELISA, respectively, in Sting or cGAS-deficient cells lines (n=3).

(F) Isg15 and phosphorylation of STAT1 at Y701 were determined in H460 cells (n=3).

(G) The plot shows the ranked p values (-log10 scaled) estimated from Wilcoxon rank test comparing gene expression levels in MSI-H versus MSS colorectal tumors from the TCGA database. The test was one-sided, to test if genes in MSI-H are expressed at higher levels than in MSS tumors. Red vertical lines marked the type I IFN induced ISGs (see Table S1 for ISGs list and expression).

Data are represented as mean ± SEM. Representative data of over 2 independent experiments are shown. Unpaired t test was used to determine significance in A-F. Wilcoxon rank sum test was used to determine significance in G.