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. Author manuscript; available in PMC: 2022 Sep 15.
Published in final edited form as: Cancer Cell. 2020 Dec 17;39(1):96–108.e6. doi: 10.1016/j.ccell.2020.11.006

Figure 4. dMLH-mediated DNA sensing promotes T-cell priming independent of TMB.

Figure 4.

(A and B) BMDCs pre-educated with MC38-OVA and B16-OVA cells were co-cultured with OT-I T cells, then T-cell proliferation was determined. IFN-γ and TNF-α were quantified. Representative FACS histograms and statistic data are shown (also see Figures S4A and S4B).

(C) Supernatants were added into co-culture system of BMDCs and OT-I T cells, then T-cell proliferation was determined. Representative FACS histograms and statistical data are shown.

(D) B16-OVA cells were inoculated into WT C57BL/6 mice (n=4). One week later, cells from the spleen were isolated and re-stimulated by OT-I peptide or control SIY peptide in vitro. T-cell responses were determined by IFN-γ ELISPOT assay.

(E and F) Fragmented tumor tissues derived from 4T1 cells with d Mlh1 (D129), double deficiency cells of Mlh1 plus Sting (D136), and double deficiency cells of Mlh1 plus cGAS (D144) were implanted into WT BALB/C mice (n=6–7). Eleven days later, tumor-infiltrating T cells were detected by FACS.

Data are represented as mean ± SEM. Unpaired t test was used to determine significance.