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Effect of GST-Bc expression when UhpA is also overexpressed. Plasmids pGEX3x-TEV and pJSW141 expressing GST or GST-Bc and plasmids pCAW8 and pA-D54N expressing UhpA or UhpA-D54N, respectively, were introduced into strain RK1306 (ΔuhpAB λuhpT-lacZ). The level of β-galactosidase was measured following growth in the absence (−) or presence (+) of Glu6P or 5 μM IPTG.
