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Effect of GST-Bc on UhpA-dependent transcription of the uhpT promoter. The conditions for in vitro transcription were 30 nM RNA polymerase, 220 nM UhpA, and 1 nM plasmid pUT1 DNA, along with the indicated final concentrations of GST or GST-Bc. The synthesis of RNA species was determined by PhosphorImager analysis of electropherograms. The locations of the uhpT-specific transcript and the vector-encoded RNAI are indicated on the right.
