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Autophosphorylation of GST-Bc variants. Purified samples of GST-Bc R324C, GST-Bc, and GST-Bc R324C H313E, each at 2.8 μM, were incubated for the indicated periods (in minutes) with [γ-32P]ATP. The samples were resolved by SDS-PAGE, the distribution of radioactivity was determined by PhosphorImager analysis (top panel), and the amount of GST-Bc protein was estimated by Coomassie blue staining of the electropherogram (bottom panel).
