TABLE 1.
E. coli strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| JM109 | e14(mcrA) recA gyrA96 thi-1 hsdR17 supE44 relA1 Δ(lac-proAB) (F′ traD36 proAB+ lacIqΔZM15) | Stratagene |
| MC4100 | Δ(argF-lac)U169 araD139 rpsL 150 relA1 flbB5301 deoC1 ptsF25 rbsR22 | |
| CW235 | MC4100 λRZ5[Km]uhpTp-lacZ recA56 srl300::Tn10 uhpAH170Y | 25 |
| RK1294 | MC4100 λRZ5[Km]uhpTp-lacZ polA1 Δuhp(A14-C437) | This work |
| RK1300 | MC4100 λRZ5[Km]uhpTp-lacZ polA1 uhp+ | |
| RK1310 | MC4100 λRZ5[Km]uhpTp-lacZ recA56 srl300::Tn10 uhp+ | |
| RK1302 | MC4100 λRZ5[Km]uhpTp-lacZ recA56 srl300::Tn10 ΔuhpB(60-489) | |
| RK1305 | MC4100 λRZ5[Km]uhpTp-lacZ recA56 srl300::Tn10 ΔuhpC(41-437) | |
| RK1306 | MC4100 λRZ5[Km]uhpTp-lacZ recA56 srl300::Tn10 Δuhp(A15-B489) | |
| RK1320 | ||
| XL-1 Blue | recA endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac (F′ proAB+ lacIq ΔZM15 Tn10) | Stratagene |
| Plasmids | ||
| pCAW8 | pACYC184 carrying uhpA coding sequence; Cm | 25 |
| pA-D54N | pCAW8 carrying uhpA D54N | This work |
| pGEX3x | lacIqlacP-gst; Ap | Pharmacia |
| pGEX3X-TEV | pGEX3X with TEV protease site | This work |
| pJSW141 | pGEX3x-TEV uhpB(273-500) and derivatives | This work |
| pRJK10 | pBR322 carrying uhpABCT; Ap | 28 |
| pSu19 | Cloning vector ori p15A from pACYC184 lac promoter; Cm | 13 |
| pSuB | pSu19 carrying lacP-(uhpA189-uhpB+-uhpC91); Cm | |
| pSuBC | pSu19 carrying lacP-(uhpA189-uhpB+-uhpC+); Cm | |
| pUT1 | pUC18 carrying uhpT promoter from −135 to +90 | 18 |