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Effect of uhpB truncation derivatives on uhp expression
| Plasmid genotype
|
β-Galactosidase activity from uhpT-lacZ reportera
|
||
|---|---|---|---|
| uhpB | uhpC | RK1310 (uhp+) (−/+) | RK1302 (ΔuhpB) (−/+) |
| Vector | 1/350 | 40/34 | |
| B+:1-500 | Δ | 1/2 | 2/3 |
| + | 1/279 | 15/262 | |
| B′:182-500 | Δ | 2/7 | 1/1 |
| + | 1/1 | 1/1 | |
| B′:288-500 | Δ | 32/108 | 115/102 |
| + | 1/213 | 1/1 | |
| B′:345-500 | Δ | 4/189 | 5/4 |
| + | 1/241 | 4/2 | |
β-Galactosidase units are presented for cells grown in the absence (−) or presence (+) of Glu6P.
