Skip to main content
. 2022 Sep 6;119(37):e2203557119. doi: 10.1073/pnas.2203557119

Fig. 5.

Fig. 5.

PNU rescues chondrogenic ability in LRP1-deficient ATDC5 cells. (A) Effects of PNU on β-catenin expression. shRNA-NC: treated with NC lentivirus. PNU (0), (10), (20): treated with shLrp1 lentivirus + 0, 10, and 20 mM PNU, respectively. β-catenin, green; DAPI, blue. (B) Relative fluorescence intensity (as a percentage) calculated for A. PNU suppressed β-catenin up-regulation due to LRP1 deficiency. (C) ATDC5 cell micromasses after chondrogenic induction for 7 d. The lentivirus-transfected shRNA-Lrp1 cells were treated with 0, 10, or 20 μM PNU for 8 d. Chondrogenic ability was evaluated by Alcian blue staining. Effects of LRP1 deficiency on chondrogenesis were rescued by PNU. (D) Western blot for β-catenin, Col2, and Sox9. The lentivirus-transfected shRNA-Lrp1 cells were treated with 0, 10, or 20 μM PNU for 8 d. Three independent experiments were assessed (n = 3). The relative protein level was determined by densitometry. Values = means ± SD; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001. (E) PNU led to a decreased level of β-catenin in both the nucleus and the cytoplasm but was much more significant in the nucleus, as detected by western blot.