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Determination of apparent Km and Vmax values of maltose transport in A. acidocaldarius cells (Lineweaver-Burk plot). Cells were grown in minimal medium in the presence of 10 mM maltose and assayed at 57°C and pH 3.6 as described in Materials and Methods. Data are from a single experiment. The Km is 1 μM, and the Vmax is 1.25 nmol/min/mg of protein.
