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Temperature (A) and pH (B) dependence of maltose binding to purified wild-type (▴) and recombinant (●) MalEAa. Binding assays were performed at the indicated temperatures in Na-acetate buffer (pH 3.6) and in the presence of 5 μM [14C]maltose. (B) Binding assays were performed at 57°C in citrate-phosphate buffer of the indicated pH. MalE of S. enterica serovar Typhimurium (■) was assayed at pH 7.4 (A) and 37°C (B). MBP, maltose binding protein.
