TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Relevant genotype or description | Source or reference |
|---|---|---|
| Strains | ||
| A. acidocaldarius ATCC 27009 | Wild type | DSMZa |
| E. coli | ||
| ED169 | F− ΔlacU169 araD139 rpsL relA thi flbB ΔmalB107 | 37 |
| PD28 | pop3325 ΔmalE444 Δ(srlR-recA)306::Tn10 | 9 |
| Top10 | F−mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 deoR recA1 araD139 Δ(araA-leu)7697 galU galK rpsL endA1 nupG | Invitrogen |
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)] | Stratagene |
| Plasmids | ||
| pBAD24 | Arabinose promoter, Apr | 17 |
| pBAD/HisA | Arabinose promoter, histidine fusion vector, Apr | Invitrogen |
| pQE9 | T5 promoter, histidine fusion vector, Apr | Quiagen |
| pSE380 | trc promoter, Apr | Stratagene |
| pSU19 | General cloning vector, P15A ori, Cmr | 33 |
| pUC18 | General cloning vector, Apr | Roche |
| pAH8-1 | ′malE as SstI-BamHI fragment on pUC18 | This study |
| pAH9 | ′malE malF malG malR′ as SstI fragment on pUC18 | This study |
| pAH10 | ′malF malG malR cdaA′ as KpnI fragment on pUC18 | This study |
| pAH19 | ′glcA pleD′ as EcoRV fragment on pUC18 | This study |
| pAH30 | ′amyA malE′ as BamHI-SstI fragment on pUC18 | This study |
| pJM30 | ′malR cdaA glcA′ as HindIII fragment on pUC18 | 34 |
| pAH18 | malEAa (Cys-1→Met) on pBAD24 | This study |
| pAH26-2 | malGAa on pBAD/HisA | This work |
| pAH27-3 | malF malGAa on pBAD/HisA | This work |
| pFSA12 | malF malGAa on pQE9 | This work |
| pFSA16 | malEEc (codons 1–26)′-′malEAa (codons 26–427) on pSE380 | This work |
| pFSA24 | pT5malF malGAa on pSU19 | This work |
a
DSMZ, Deutsche Sammlung von Mikroorganismen und Zellkulturen.