Skip to main content
. 2022 Sep 2;18(9):e1010362. doi: 10.1371/journal.pgen.1010362

Fig 3. Increased 1197 p-EGFR in H23 PTPRH CRISPR KO Cells.

Fig 3

Western blotting using lysate from PTPRH KO cells and PTPRH KO cells with transient overexpression of PTPRH plasmids demonstrates PTPRH indeed targets EGFR within human lung cancer cells. A) Electropherogram of PTPRH KO clones shows an A insertion at the CRISPR cut site. This indel was present for both clones in 3B. B) PTPRH KO CRISPR clones have increased 1197 phosphorylated EGFR. C) Overexpression of a wild type PTPRH plasmid within PTPRH KO clone 1 reduced 1197 p-EGFR. D) Overexpression of a D986A mutant PTPRH plasmid within PTPRH KO clone 1 resulted in no reduction of 1197 p-EGFR. E) Increased p-AKT is seen within 1 of the PTPRH KO clones, but not the other. F) To investigate potential clonal effects further, we created a Y1197F EGFR mutation within H23 PTPRH KO clone 1. G) Step-wise reduction of 1197 EGFR phosphorylation is seen within heterozygous and homozygous Y1197F clones. These Y1197F clones also have marked reduction in p-AKT.