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. 2022 Sep 2;18(9):e1010362. doi: 10.1371/journal.pgen.1010362

Fig 6. PTPRH Regulation of Receptor Tyrosine Kinases outside the ERBB Family.

Fig 6

Ablation of PTPRH results in differential activation of numerous receptor tyrosine kinases outside of EGFR. A) A human phosphorylated RTK array shows differential phosphorylation of numerous RTKs when incubated with lysate from H23 PTPRH WT cells. B) A human phosphorylated RTK array shows differential phosphorylation of numerous RTKs when incubated with lysate from H23 PTPRH KO cells. Top five differentially phosphorylated RTKs are highlighted in red. C) Table showing the RTKs with the top 5 largest fold changes between PTPRH KO and PTPRH WT lysates. D) The lollipop plot in Fig 2B was recreated, adding predicted activation for FGFR1 and IGF-1R. Briefly, each dot on the PTPRH exome plot corresponds to a PTPRH mutant NSCLC tumor. Color-coded bars above each dot correspond to the predicted activity of EGFR, FGFR1, or IGF-1R. E) Western blotting confirms increased phosphorylation of FGFR1 at tyrosine residues 653/654, within PTPRH KO cell lysates compared to PTPRH WT lysates.