Lcn2-/- knockout mice and their WT C57BL/6 littermate controls were infected retro-orbitally with WT A. baumannii. The infection was allowed to proceed for 24 h before the mice were humanely euthanized and the organs were harvested. Organs were homogenized, serially diluted in PBS and plated to Luria-Bertani agar (LBA). The bacterial burdens of the kidneys (A), heart (B), liver (C), spleen (D), lungs (E) and blood (F) were determined. Each symbol represents the WT A. baumannii count in the corresponding organ of one animal. Data are compiled from two independent experiments. Statistical significance was determined by Mann-Whitney U test, where ns = not significant. The dashed line represents the limit of detection for the assay.