Table 1. Strains employed in this study.
Strain | Description1 | Reference |
---|---|---|
A. baumannii ATCC 17978 UN | WT A. baumannii from fatal pediatric meningitis, UN isolate. Reference genome CP079931. | [172,174] |
A. baumannii ATCC 17978 VU | WT A. baumannii from fatal pediatric meningitis, VU isolate. Reference genomes NZ_CP018664; CP012004 | [172,174–176] |
ΔbasG | Acinetobactin and pre-acinetobactin biosynthetic mutant; markerless | [19] |
ΔbfnL | Baumannoferrin A and B biosynthetic mutant; markerless | [19] |
ΔfbsE | Fimsbactins A through F biosynthetic mutant; markerless | [19] |
ΔbasG bfnL | Acinetobactin and baumannoferrins biosynthetic mutant. Encodes for the fimsbactins; KanR | [19] |
ΔbasG fbsE | Acinetobactin and fimsbactins biosynthetic mutant. Encodes for the baumannoferrins; KanR | [19] |
ΔbfnL fbsE | Baumannoferrins and fimsbactins biosynthetic mutant. Encodes for acinetobactin and pre-acinetobactin; KanR | [19] |
ΔbasG bfnL fbsE | Acinetobactin and pre-acinetobactin, baumannoferrins, and fimsbactins biosynthetic mutant. Siderophore-deficient; KanR | [19] |
WT A. baumannii VU/ p.PfbsB.luxABCDE.MU368.tet | WT A. baumannii containing the p.luxABCDE.MU368.tet plasmid with the iron-regulated fbsB promoter cloned upstream of luxABCDE; TetR | This study |
WT A. baumannii VU/ p.luxABCDE.MU368.tet | WT A. baumannii containing the promoterless p.luxABCDE.MU368.tet plasmid construct; TetR | [25] |
1Strains marked KanR have the last gene in their strain designation replaced with a kanamycin resistance determinant.