Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus, females) | C57BL/6 J | The Jackson Laboratory | RRID:IMSR_JAX:000664 | |
| Strain, strain background (Mus musculus, males) | MLL-AF9 | The Jackson Laboratory | RRID:IMSR_JAX:009079 | Wild type littermates used as controls |
| Cell line (Homo sapiens) | SKM-1 | DMSZ | DMSZ ACC 547; RRID: CVCL_0098 | |
| Cell line (Homo sapiens) | Kasumi-1 | DMSZ and a gift from the laboratory of C.Bonifer (UoB) |
DMSZ ACC 220; RRID: CVCL_0589 | |
| Cell line (Homo sapiens) | HL-60 | ATCC | ATCC CCL-240 RRID: CVCL_0002 |
|
| Cell line (Mus musculus) | MS-5 | Gift from the laboratory of JJ Schuringa (Groninger University) |
DMSZ ACC 441 RRID:CVCL_2128 |
|
| Cell line (Homo sapiens) | HeLa | ATCC | ATCC CCL-2 RRID: CVCL_0030 |
|
| Antibody | CD33 (mouse monoclonal, clone P67.6) | eBioscience | 48-0337-42 | 1:100 |
| Antibody | anti-CD34 APC (mouse monoclonal) | BD Biosciences | 560940 | 10 ul per 10 million cells |
| Antibody | anti-CD38 FITC (mouse monoclonal) | BD Biosciences | 555459 | 10 ul per 10 million cells |
| Chemical compound, drug | tert-Butyl hydroxyperoxide (TBHP) | Sigma/Merck | 478139 | |
| Chemical compound, drug | carbenoxolone (CBX) | Sigma-Aldrich | C4790 | For in vitro experiments |
| Chemical compound, drug | carbenoxolone (CBX) | Thermofisher | J63714.03 | For in vivo experiments |
| Chemical compound, drug | Catalase | Sigma-Aldrich | C100 | |
| Chemical compound, drug | 2′,7′-Dichlorofluorescin diacetate (DCFH-DA) | Sigma-Aldrich | D6883 | |
| Chemical compound, drug | H2O2 | Merck | 386790 | |
| Chemical compound, drug | N-acetylcysteine (NAC) | Merck | 106425 | |
| Chemical compound, drug | Calcein-AM green | Invitrogen | C1430 | |
| Chemical compound, drug | CellTrace carboxyfluorescein succinimidyl ester (CFSE) | Invitrogen | C34570 | |
| Chemical compound, drug | ACSS2i | Selleck | S8588 | |
| Chemical compound, drug | Cytarabine (AraC) | Sigma-Aldrich | C1768 | |
| Chemical compound, drug | [U-13C]Glucose | CortecNet | CC860P1 | |
| Chemical compound, drug | [2-13C]acetate | Sigma-Aldrich | 279315–1 G | |
| Other | CD34 magnetic microbeads | Miltenyi Biotec | 130-046-702 | Please see Materials and Methods section under Primary Patient samples |
| Other | CD117 magnetic microbeads | Miltenyi Biotec | 130-091-332 | Please see Materials and Methods section under Primary Patient samples |
| Sequence-based reagent | Hk2 q PCR primers | Sigma-Aldrich | NM_013820 | KiCqStart primers KSPQ12012 |
| Sequence-based reagent | Pdhx q PCR primers | Sigma-Aldrich | NM_175094 | KiCqStart primers KSPQ12012 |
| Sequence-based reagent | Pdk1 q PCR primers | Sigma-Aldrich | NM_172665 | KiCqStart primers KSPQ12012 |
| Sequence-based reagent | Pdk2 q PCR primers | Sigma-Aldrich | NM_133667 | KiCqStart primers KSPQ12012 |
| Sequence-based reagent | B2m qPCR primers | Thermo Fisher Scientific | NM_009735.3 | |
| Software.algorithm | Flowjo | BD-Bioscience | ||
| Software.algorithm | MetaboLab software within the MATLAB environment (MathWorks). |
Ludwig and Günther, 2011 . https://doi.org/10.1186/1471-2105-12-366 |
https://www.ludwiglab.org/software-development | |
| Software.algorithm | Chenomx 7.0 software (Chenomx Inc) | Chenomx Inc https://www.chenomx.com/ |
||
| Software.algorithm | FastQC 0.11.7 software | http://www.bioinformatics.babraham.ac.uk/projects/fastqc/ | ||
| Software.algorithm | Kallisto 0.43.0 software | Bray et al., 2016 | https://pachterlab.github.io/kallisto/ | |
| Software.algorithm | R statistical package Sleuth 0.30.0 | Pimentel et al., 2017 | https://github.com/pachterlab/sleuth | |
| Software.algorithm | the R statistical package fgsea 1.10.0 | http://bioconductor.org/packages/release/bioc/html/fgsea.html | ||
| Software.algorithm | R statistical package BioMart 2.40.3 | https://bioconductor.org/packages/biomaRt | ||
| Software.algorithm | GraphPad version 8 | https://www.graphpad.com/ |